Beschreibung
InhaltsangabePart I: Genetics, Cytology, and In Vivo Analysis 1. Screening or Selection for Chloroplast Biogenesis Mutants of Arabidopsis, Following Chemical or Insertional Mutagenesis Enrique López-Juez and Alison Hills 2. Analysis of Plastid Number, Size, and Distribution in Arabidopsis Plants by Light and Fluorescence Microscopy Kevin Pyke 3. Immunofluorescence Microscopy for Localization of Arabidopsis Chloroplast Proteins Stanislav Vitha and Katherine W. Osteryoung 4. Transient Expression and Analysis of Chloroplast Proteins in Arabidopsis Protoplasts Dong Wook Lee and Inhwan Hwang 5. Visualisation of Stromules on Arabidopsis Plastids John C. Gray, James A. Sullivan, and Christine A. Newell 6. Analysis of Chloroplast Movement and Relocation in Arabidopsis Masamitsu Wada and Sam-Geun Kong 7. Studying Starch Content and Sedimentation of Amyloplast Statoliths In Arabidopsis Roots John Stanga, Allison Strohm, and Patrick H. Masson 8. Studying Arabidopsis Chloroplast Structural Organization Using Transmission Electron Microscopy Stefan Hyman and R. Paul Jarvis 9. Transplastomics in Arabidopsis: Progress Towards Developing an Efficient Method Kerry Ann Lutz, Arun Azhagiri, and Pal Maliga Part II: Gene Expression and Protein Accumulation 10. Isolation, Quantification, and Analysis of Chloroplast DNA Beth A. Rowan and Arnold J. Bendich 11. Measurement of Transcription Rates in Arabidopsis Chloroplasts Yan O. Zubo, Thomas Börner, and Karsten Liere 12. Studying the Structure and Processing of Chloroplast Transcripts Alice Barkan 13. In Vitro RNA Binding Assay for Studying Trans-Factors for RNA Editing in Chloroplasts Toshiharu Shikanai and Kenji Okuda 14. Studying Translation in Arabidopsis Chloroplasts Paolo Pesaresi 15. Studying Proteases and Protein Turnover in Arabidopsis Chloroplasts Lars L.E. Sjögren and Adrian K. Clarke Part III: Protein Transport, Localization, and Topology 16. In Silico Methods for the Identification of Organellar and Suborganellar Targeting Peptides in Arabidopsis Chloroplast Proteins and for Predicting the Topology of Membrane Proteins Sandra Tanz and Ian Small 17. Rapid Isolation of Arabidopsis Chloroplasts and Their Use for In Vitro Protein Import Assays Henrik Aronsson and R. Paul Jarvis 18. Energetic Manipulation of Chloroplast Protein Import and the Use of Chemical Cross-Linkers to Map Protein-Protein Interactions Hitoshi Inoue, Fei Wang, Takehito Inaba, and Danny J. Schnell 19. Isolation of Arabidopsis Thylakoid Membranes and Their Use for In Vitro Protein Insertion or Transport Assays Thomas Bals and Danja Schünemann 20. Determining the Location of an Arabidopsis Chloroplast Protein Using In Vitro Import Followed by Fractionation and Alkaline Extraction Chiung-Chih Chu and Hsou-min Li 21. Studying Arabidopsis Envelope Protein Localization and Topology Using Thermolysin and Trypsin Proteases John Froehlich
Inhalt
Part I: Genetics, Cytology, and In Vivo Analysis 1. Screening or Selection for Chloroplast Biogenesis Mutants of Arabidopsis, Following Chemical or Insertional Mutagenesis Enrique Lopez-Juez and Alison Hills 2. Analysis of Plastid Number, Size, and Distribution in Arabidopsis Plants by Light and Fluorescence Microscopy Kevin Pyke 3. Immunofluorescence Microscopy for Localization of Arabidopsis Chloroplast Proteins Stanislav Vitha and Katherine W. Osteryoung 4. Transient Expression and Analysis of Chloroplast Proteins in Arabidopsis Protoplasts Dong Wook Lee and Inhwan Hwang 5. Visualisation of Stromules on Arabidopsis Plastids John C. Gray, James A. Sullivan, and Christine A. Newell 6. Analysis of Chloroplast Movement and Relocation in Arabidopsis Masamitsu Wada and Sam-Geun Kong 7. Studying Starch Content and Sedimentation of Amyloplast Statoliths In Arabidopsis Roots John Stanga, Allison Strohm, and Patrick H. Masson 8. Studying Arabidopsis Chloroplast Structural Organization Using Transmission Electron Microscopy Stefan Hyman and R. Paul Jarvis 9. Transplastomics in Arabidopsis: Progress Towards Developing an Efficient Method Kerry Ann Lutz, Arun Azhagiri, and Pal Maliga Part II: Gene Expression and Protein Accumulation 10. Isolation, Quantification, and Analysis of Chloroplast DNA Beth A. Rowan and Arnold J. Bendich 11. Measurement of Transcription Rates in Arabidopsis Chloroplasts Yan O. Zubo, Thomas Börner, and Karsten Liere 12. Studying the Structure and Processing of Chloroplast Transcripts Alice Barkan 13. In Vitro RNA Binding Assay for Studying Trans-Factors for RNA Editing in Chloroplasts Toshiharu Shikanai and Kenji Okuda 14. Studying Translation in Arabidopsis Chloroplasts Paolo Pesaresi 15. Studying Proteases and Protein Turnover in Arabidopsis Chloroplasts Lars L.E. Sjögren and Adrian K. Clarke Part III: Protein Transport, Localization, and Topology 16. In Silico Methods for the Identification of Organellar and Suborganellar Targeting Peptides in Arabidopsis Chloroplast Proteins and for Predicting the Topology of Membrane Proteins Sandra Tanz and Ian Small 17. Rapid Isolation of Arabidopsis Chloroplasts and Their Use for In Vitro Protein Import Assays Henrik Aronsson and R. Paul Jarvis 18. Energetic Manipulation of Chloroplast Protein Import and the Use of Chemical Cross-Linkers to Map Protein-Protein Interactions Hitoshi Inoue, Fei Wang, Takehito Inaba, and Danny J. Schnell 19. Isolation of Arabidopsis Thylakoid Membranes and Their Use for In Vitro Protein Insertion or Transport Assays Thomas Bals and Danja Schünemann 20. Determining the Location of an Arabidopsis Chloroplast Protein Using In Vitro Import Followed by Fractionation and Alkaline Extraction Chiung-Chih Chu and Hsou-min Li 21. Studying Arabidopsis Envelope Protein Localization and Topology Using Thermolysin and Trypsin Proteases John Froehlich
